Yeast cell wall breaking high shear colloid mill

Yeast cell grinding emulsifier, yeast cell high-speed grinding disperser, cell wall breaking grinding disperser, laboratory grinding disperser, high shear homogenizer, high shear emulsifier

Cell lysis, also known as cell lysis technology, refers to the use of external forces to break down the cell membrane and cell wall, resulting in the release of cellular contents, including target product components. When cell lysis, separation, and purification of a certain protein, the first step is to release the protein from the tissue or cell and maintain its original natural state without losing its activity. So it is necessary to use appropriate methods to break down tissues and cells. Different organisms or tissues from different parts of the same organism have varying degrees of difficulty and methods of cell breakage. For example, the cell membrane of animal organs is fragile and prone to breakage, while plants and microorganisms have cell walls composed of strong cellulose and hemicellulose.

The inner layer of yeast cell wall is composed of fine fibers of pectin, which forms the rigid skeleton of the cell wall and gives the cell a certain shape. A layer of glycoprotein covers the fine fibers, and the outer layer is mannan, which is covalently connected by 1,6-phosphate diester bonds to form a network structure. Inside this layer, there is a complex of mannan enzyme, which can be covalently attached to the network structure or not. Like bacterial cell walls, the resistance to breaking yeast cell walls is mainly determined by the degree of cross-linking in the wall structure and its thickness.

The yeast cell crushing, grinding and dispersing machine is a high-speed (9000prm) high shear homogenizer specially developed for cell wall breaking. With a special split structure, the 316L stainless steel stator and rotor generate strong shear force, and the kicking and tearing forces between materials can make the particle size of cells reach the nanometer and micrometer level. This greatly improves the cell wall breaking rate and enhances the extraction efficiency.

The Taicang Xide cell wall breaking homogenizer has incomparable advantages compared to traditional crushing processes:
The traditional crushing process for medicinal herbs has a particle size of 150-200 microns, which cannot achieve cell wall breaking. The Sid mechanical cell wall breaking homogenizer can achieve a particle size of 1-5 microns for medicinal materials, with a wall breaking rate of over 95%;
2. The traditional crushing process has a large particle size distribution range and poor uniformity, which affects the stable clinical efficacy. The SID cell wall breaking homogenizer reduces the particle size distribution range of medicinal materials, makes the particle size more uniform, and improves the stability of product quality and clinical efficacy;
3. Traditional crushing techniques cannot achieve low-temperature crushing, and equipment temperatures can reach up to 80 degrees, causing denaturation of some protein active ingredients in medicinal materials and affecting therapeutic efficacy. The SID cell wall breaking homogenizer adopts the Baumann machine sealing system, which can be carried out under conditions of 0-50 degrees, improving the stability of protein thermosensitive active ingredients in animal medicinal materials and further enhancing clinical efficacy.

The grinding emulsifier is a high-tech product composed of a colloid mill disperser.

The first level consists of three-level sawtooth protrusions and grooves with increasing precision. The stator can be infinitely adjusted to the desired distance between the rotors. Under enhanced fluid turbulence. The groove can change direction at each level.
The second stage is composed of a stator. The design of the dispersing head also effectively meets the needs of substances with different viscosities and particle sizes. The difference in the design of the stator and rotor (emulsifying head) between online and batch machines is mainly due to the requirements for conveying performance. It is particularly important to note that the difference between coarse precision, medium precision, fine precision, and other types of working heads is not only the arrangement of specified rotor teeth, but also an important difference in the geometric characteristics of different working heads. The width of the slot and other geometric features can alter the different functions of the stator and rotor working heads.

The following is a model table for reference:

Yeast cell wall breaking high shear colloid mill